locus

BSU08080

BSU_08080

geneLength

1194

1197

outlinks

bsu

BSU08080

BSU_08080

The protein

Catalyzed reaction/ biological activity

Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine (according to Swiss-Prot)

(R)-N⁶-dihydrolipoyl-L-lysyl-[protein] + acetyl-CoA --> (R)-N⁶-(S⁸-acetyldihydrolipoyl)-L-lysyl-[protein] + CoA (according to UniProt)

The protein

Protein family

lipoyl-binding domain (according to Swiss-Prot)

[SW|2-oxoacid dehydrogenase family] (according to UniProt)

The protein

Paralogous protein(s)

[[protein|BkdB]], [[protein|OdhB]], [[protein|PdhC]]

[[this]]

Biological materials

Mutant

MGNA-C282 (acoC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2280 NBRP B. subtilis, Japan]

BKE08080 (Δ[[gene|acoC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTACCGCCATTTGTGTCC, downstream forward: _UP4_TAGGAAAAGCAGGTGAAAAC

BKK08080 (Δ[[gene|acoC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTACCGCCATTTGTGTCC, downstream forward: _UP4_TAGGAAAAGCAGGTGAAAAC

Labs working on this gene/protein

[SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]

References

Original publications

11274109, 10368162, 10666464, 16479537, 16428414, 12884008, 21815947

11274109, 10368162, 10666464, 16479537, 16428414, 12884008, 21815947, 19081062, 31066113

The protein

[SW|Domains]

[SW|Lipoyl-binding domain] (aa 2-77) (according to UniProt)

[SW|Peripheral subunit-binding domain] (PSBD) (aa 118-155) (according to UniProt)

The protein

Structure

[PDB|3DUF] (PDH from Geobacillus stearothermophilus, 33% identity) [pubmed|19081062]

Expression and Regulation

additional information

the mRNA is very stable (> 15 min) [pubmed|12884008]

labs

[SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]